Chemistry DNA Extraction from Kiwi Fruit   DNA extraction from a Kiwi fruit     Subject Chemistry Level Upper secondary Language English Type Experiment       Experiment   A simple protocol to see and touch real DNA: cut the kiwi (1/2 big or 1 small) into small pieces take the table salt, the washing-up liquid, and the distilled water and mix it well in a beaker add the chopped kiwi wait for 10 minutes blend the mixture (with a blender) for exactly 5 seconds filter the mixture into a second beaker add 10 drops of protease (meat tenderizer or contact lens cleaning liquid) and mix pour 20 ml of the filtrate into a large test tube very carefully pour ice-cold ethanol (95%) down the side of the tube to form a layer on top of the kiwi extract collect the filaments of DNA wrapping them around a inoculation loop.     Contact details   Name Immacolata Ercolino Giulia Realdon Email immacolata.ercolino@istruzione.it giulia.realdon@virgilio.it       Last update: 14 December 2006 More Chemistry Teaching Ideas  •  How does Toothpast work? (http://www.esa.int/SPECIALS/Science_on_Stage/SEMC6RC4VUE_0.html)  •  Teaching Science and Humanities (http://www.esa.int/SPECIALS/Science_on_Stage/SEMBXOC4VUE_0.html)  •  ICT in Chemistry (http://www.esa.int/SPECIALS/Science_on_Stage/SEMTNY9RR1F_0.html)  •  The Particularities of Chemistry Teaching in Luxembourg (http://www.esa.int/SPECIALS/Science_on_Stage/SEMC41ARR1F_0.html)  •  Web Chemistry: Sweeteners (http://www.esa.int/SPECIALS/Science_on_Stage/SEM4P2ARR1F_0.html)  •  The Shadow of the Flame (http://www.esa.int/SPECIALS/Science_on_Stage/SEMZ93ARR1F_0.html)  •  Looking for Antioxidant Food (http://www.esa.int/SPECIALS/Science_on_Stage/SEM3F4ARR1F_0.html)  •  Discovering an Accessible Enzyme (http://www.esa.int/SPECIALS/Science_on_Stage/SEM0O4ARR1F_0.html)  •  Natural Antibiotics (http://www.esa.int/SPECIALS/Science_on_Stage/SEMOW4ARR1F_0.html)  •  Some Simple Demonstrations in Classroom (http://www.esa.int/SPECIALS/Science_on_Stage/SEMJFC8OY2F_0.html) Download  •  DNA extraction PDF (http://esamultimedia.esa.int/docs/scienceonstage/DNA_Extraction_from_Kiwi_Fruit_SOS1.pdf)