Experiment design

a Animals were amputated the day before of the exposure to simulated launch loads (day −1). At day 0, animals were loaded into T25 flasks and the experiment was initiated. Immediately after the exposure to hypergravity, vibration or both, RNA was extracted from half of the animals (0 h). Four days after the exposure, the RNA of the rest of the animals was extracted (4d). The regenerated structures were imaged at 4 days after the exposure. b The four experimental groups of animals. See de Sousa, N., Caporicci, M., Vandersteen, J. et al. Molecular impact of launch related dynamic vibrations and static hypergravity in planarians. npj Microgravity 6, 25 (2020). https://doi.org/10.1038/s41526-020-00115-7